Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida

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Effect of jasmonic acid and aluminum on production of tropane alkaloids in hairy root cultures of Brugmansia candida

Hairy root cultures of Brugmansia candida (Solanaceae), a South American plant which produces scopolamine and hyoscyamine, were exposed to different elicitors (jasmonic acid (JA) and aluminum chloride (AlCl3)) in order to increase their productivity and/or stimulate their liberation. Hairy roots of 19-day old cultures (exponential phase) were exposed to these elicitors for 24 and 48 hours. The ...

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Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

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effect of elicitation on antioxidant activity and production of tropane alkaloids in hyoscyamus reticulatus hairy root cultures

background and objectives: hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. in the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in hyoscyamus reticulatus. methods: effect of different factors including agrobacteriu...

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Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae)

Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologicallyimportant group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-β hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6h...

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ژورنال

عنوان ژورنال: Electronic Journal of Biotechnology

سال: 2000

ISSN: 0717-3458,0717-3458

DOI: 10.2225/vol3-issue1-fulltext-6